Bio-Service

Protein identity certification experiment service

The service features the utilization of LC-MS/MS mass spectrometer for ascertaining protein identity. Following treatment of enzymatic hydrolysis, every peptide of protein undergoes series mass-spectrometer analysis before ascertaining protein identity via database comparison. The analysis is carried out using peptide as unit and can be carried out for a single protein or mixed protein. The service can cover complete series of experiment, including surrogate electrophoresis, sampling, hydrolysis of protein, mass-spectrometer analysis, and database comparison. In addition to identify ascertaining, services for scanning, positioning, and  of up to 100 post-translation modifications (PTMs), and proof and analysis of mass spectrum are also available.

Service code No. Service title Explanation Service contents Expected time
SV03

Experimental service for protein identity certification

 Utilize LCMSMS for peptide analysis and carry out protein comparison and identification with database
  1. Protein hydrolysis
  2. LCMSMS mass spectrometer analysis
  3. Open database for self identity comparison via database analysis
 Two to three weeks
SV04

Experimental service for post-translation protein modification and scanning

 Carry out various post-translation modification and analysis for amino acid in peptide and ascertain kinds and sequencing of post-translation modification
  1. Protein hydrolysis
  2. LCMSMS mass spectrometer analysis
  3. Open database for self identity comparison via database analysis
  4. Post-translation modification, scanning, and analysis
 Two to three weeks
SV05 Experimental service for post-translation modification of protein position map Carry out post-translation modification of map position for amino acid in peptide
  1. Protein hydrolysis
  2. LCMSMS mass spectrometer analysis
  3. Open database for self identity comparison via database analysis
  4. Post-translation modification, scanning, and analysis
  5. Post-modification map establishment and analysis
 Two to three weeks

 

Analysis for the service employs LCMSMS, an LTQ Oribitrap model, featuring high precision in identifying peptide, thanks to the use of ion-trap channel, facilitating the analysis for determining the size and sequence of peptide molecule, on top of comparative analysis for 100 kinds of post-translation modifications, resulting in post-modification sequence and map.

 

 

 

 

 

 

 

 

 

Service flow​

  1. Customer consulting: We will discuss with you on experiment and offer a quote.
  2. Delivery of specimen: Customer can notify the company to collect specimen or deliver it to the company via courier.
  3. Confirmation of specimen quality: Specimen will be first subject to quality confirmation, to assure their suitability for experiment.
  4. Execution of experiment: During the process of experiment, customers can inquire about its progress.
  5. Delivery of service report: After completion of experiment, a service report and related materials will be delivered to customers.

 

Notice for service​

  • For any questions on the service, please contact the company's Life Science Service Division at (Tel.: 886-2-26270835，Email：service@visualprotein.com) for discussion on details of your needs.

 

Notices for delivery of specimen​

 

  • Please download the document "order for Protein identity certification and systematic biological  experiment service for specimen" and fill in service needs and related information on specimen, as basis for the company to carry out the service.
  • Please download and read the document "notices for preparation and delivery of specimen" before preparing specimen for analysis, along with the service order, before notifying the company to collect them or delivering them via courier to the company at Tel: 886-2-26270835, zip code: 11492, address: Fl., 6-3, No. 21, lane 583, Ruiguang Road, Neihu, Taipei, Biotech Service Division, Energenesis Biomedical)