Protein Quantification

Dual-Range Bradford Protein Assay

Dual-Range™ Bradford Protein Assay is a fast protein quantification method. It based on the method of Bradford, coomassie-binding with protein in an acidic solution. The measurement of absorbance shifts from 465 nm (brown color) to 595 nm (blue color) when binding to protein occurs. In addition, the coloration differs greatly depending on the basic and aromatic amino acid residues of protein. Dual-Range™ Bradford Protein Assay provides a wide protein quantification range from 1-1,000 μg/mL and the measured absorbance at 595 nm is stable for 5 to 60 minutes after the binding reaction starts.


Dual-Range BCA Protein Assay

Dual-Range™ BCA Protein Assay Kit is the most used protein assay method. It based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein in the 5–2,000 μg/mL concentration range. Like the Lowry method, the assay relies on the reduction of Cu2+ ions by protein. The Cu+ thus formed is detected by conversion into a violet-colored substance by reaction with bicinchoninate. Dual-Range™ BCA Protein Assay Kit is compatible with many detergents but not compatible with reducing agents such as DTT,DTE, and 2-Mercaptoethanol etc.